Peculiarities of the Biological Effects Formation in Cultured Human Tumor A549 Cells Exposured to Ionizing Radiation with Different Dose Rates
https://doi.org/10.56304/S2304487X22030105
Abstract
The influence of the dose rate factor of ionizing radiation on the induction and repair efficiency of double-strand breaks (DSBs) of DNA has been studied with further prognostic analysis of the survival of human tumor cells. A549 cells have been exposed to X rays at a dose of 2 Gy at dose rates of 10 and 400 mGy/min. The degree of DNA fragmentation, immunocytochemical staining for γH2AX and RAD51 proteins and clonogenic test have been conducted. It has been found that the DNA DSB repair velocity under irradiation with a lower dose rate (10 mGy/min) is lower than that under irradiation with a high dose rate (400 mGy/min) and predominantly passes through the mechanism of homologous recombination. Other DNA DSB repair mechanisms predominate in cells irradiated at a higher dose rate (400 mGy/min). The clonogenic ability of tumor cells after irradiation with the used dose rates (10 and 400 mGy/min) decreases significantly compared with non-irradiated cells, but no significant differences have been observed between cells irradiated with different dose rates.
About the Authors
V. A. Stelmukhova
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Russian Federation
Russian Federation
Moscow
115409
123098
A. A. Tsishnatti
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Russian Federation
Russian Federation
Moscow
115409
123098
S. M. Rodneva
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Russian Federation
Russian Federation
Moscow
123098
N. M. Smetanina
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Russian Federation
Russian Federation
Moscow
123098
Y. A. Fedotov
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Russian Federation
Russian Federation
Moscow
123098
D. V. Guryev
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency
Russian Federation
Russian Federation
Moscow
123098
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Review
For citations:
Stelmukhova V.A., Tsishnatti A.A., Rodneva S.M., Smetanina N.M., Fedotov Y.A., Guryev D.V. Peculiarities of the Biological Effects Formation in Cultured Human Tumor A549 Cells Exposured to Ionizing Radiation with Different Dose Rates. Vestnik natsional'nogo issledovatel'skogo yadernogo universiteta "MIFI". 2022;11(3):209–217. (In Russ.) https://doi.org/10.56304/S2304487X22030105
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