EXPERIMENTAL STUDY OF THE SUPRAMOLECULAR SERUM ALBUMIN STRUCTURE
https://doi.org/10.26583/vestnik.2023.284
EDN: BPBNME
Abstract
The paper presents the results of experimental studies of water-salt solutions of serum albumin (SAC), which is considered as an analogue of body fluids. The observed structural effects in dehydrated films are considered taking into account the concept of the supramolecular organization of the polymer body and its multilevel structure. To study the samples, a single complex technique was developed and tested, including studies of the supramolecular structure of the entire drop at the optical level (microlevel) and the morphology of the globular structural elements of the albumin film (nanolevel) by atomic force microscopy (AFM). Processing of SAC facies with the help of the Morpho hardware and software complex allowed us to establish the dependence of the NM facies on the albumin concentration. At the optical level, it was possible to observe a complex-step mechanism of changes in the supramolecular structure of water-salt facies of albumin. The study showed that with an increase in protein concentration, the area of the crystallization area of dendrites decreases. At a concentration of 10 % SAC, a structural transition occurs: the dendritic-fractal morphology is replaced by a system of cracks and individual nodules. The globular structure of the protein matrix was revealed by atomic force microscopy. The size of a single globule and protein associates, and the density of their packaging, depending on the concentration, were estimated.
About the Authors
A. V. Volkova
SarPhTI MEPHI
Russian Federation
Russian Federation
M. E. Buzoverya
FSUE RFNC-VNIIEF
Russian Federation
Russian Federation
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Review
For citations:
Volkova A.V., Buzoverya M.E. EXPERIMENTAL STUDY OF THE SUPRAMOLECULAR SERUM ALBUMIN STRUCTURE. Vestnik natsional'nogo issledovatel'skogo yadernogo universiteta "MIFI". 2023;12(5):255-261. (In Russ.) https://doi.org/10.26583/vestnik.2023.284. EDN: BPBNME
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